ABSTRACT
<p><b>OBJECTIVE</b>To investigating the relation between the expression of P-glycoprotein and Glutathione transferase-pi and the chemoresistance.</p><p><b>METHODS</b>The expressions of these two proteins in patients with oral and maxillofacial squamous carcinoma and normal oral tissues were detected by immunohistochemistry.</p><p><b>RESULTS</b>The positive expression rate of P-gp and GST-pi in oral and maxillofacial malignant tumor was 57.1% and 53.6% respectively, and no expression in normal oral tissues; the expression of GST-pi was relevant to the resistance to cisplatin, while the expression of P-gp was relevant to the resistance to chemotherapeutic drug in general.</p><p><b>CONCLUSIONS</b>The method of immunohistochemistry combining MTT assay in vitro may become an efficient way to predict the sensitivity to chemotherapeutic drug.</p>
Subject(s)
Humans , ATP Binding Cassette Transporter, Subfamily B, Member 1 , Carcinoma, Squamous Cell , Chemistry , Drug Therapy , Drug Resistance, Neoplasm , Facial Neoplasms , Chemistry , Drug Therapy , Formazans , Glutathione S-Transferase pi , Glutathione Transferase , Immunohistochemistry , Isoenzymes , Maxillary Neoplasms , Chemistry , Drug Therapy , Mouth Neoplasms , Chemistry , Drug Therapy , Tetrazolium SaltsABSTRACT
<p><b>OBJECTIVE</b>To investigate whether paclitaxel (Taxel) can efficiently induce apoptosis of ACC-2 or not, and to study the relation of apoptosis and arrest of cell mitosis.</p><p><b>METHODS</b>Paclitaxel-induced arrest of cell mitosis and apoptosis of ACC-2 cells in various concentration and different treat time were determined using transmission electron microscope (TEM), fluorescence microscope, flow-cytometry and DNA agarose gel electrophoresis technique.</p><p><b>RESULTS</b>Under fluorescence microscope, apoptotic cells were green with irregular clumping of nucleus chromatin, or even nuclear chromatin segregation. The typical ultra-structural changes of apoptosis observed by TEM were cell compaction, margination of nuclear chromatin, condensation of cytoplasm, protuberances and apoptotic body. "DNA Ladder" was absent in agarose gel electrophoresis of DNA extracted from culture of ACC-2 cells and paclitaxel-induced ACC-2 cells. "Sub-G(1)" phase peak of ACC-2 cells induced by 50 nmol/L paclitaxel in 48 h and 72 h was 17.13% and 16.26%, respectively. The percentage of G(2)/M phase increased in accordance with raise of the paclitaxel concentration and prolongation of treatment. The typical ultra-structural changes of apoptosis were observed in case that G(2)/M phase was arrested.</p><p><b>CONCLUSIONS</b>Paclitaxel could induce apoptosis of ACC-2 cells. Arrest of G(2)/M phase might induce apoptosis of ACC-2 cells.</p>